ABSTRACT
Objective: To investigate the interspecific relationships among thirteen species (include two varieties) of Lycoris Herb, from different habitats and provide the evidence for evaluation and exploitation of the germplasm resources for Lycoris Herb. Methods: The genetic diversities of 20 accessions of Lycoris Herb, from different habitats were investigated with the technique of amplified fragment length polymorphism (AFLP). Results: Six pairs of AFLP primers were selected from 33 pairs of combinations, and a total of 330 amplified clear DNA bands were obtained, among them 320 were polymorphic bands. The percentage of polymorphic bands was 96.8%. The coefficient ranges of interspecific genetic similarity (GS) in AFLP analyses for the 20 materials were 0.38-0.89. AFLP clustering results showed that the materials from the same habitats had a closer genetic relationship. There were abundant genetic diversity in species of Lycoris Herb. Conclusion: AFLP technique is efficient in generating more accurate information on the genetic diversity and relationship in plants of Lycoris Herb.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Fuzheng Jiangan formula( FZJGF) on liver fibrosis using immune induced liver fibrosis rat model.</p><p><b>METHOD</b>The rat models with immunity liver fibrosis were induced by the human serum albumin. Rats were treated with normal saline, FZJGF (9. 85,39. 4 g x kg(-1) , two dosage groups) and Colchicine (0. 000 1 g . kg(-1) ). The activities of ALT, AST, contents of ALB and TP, and A/G, The contents of Laminin (LN), Hyaluronic acid (HA) and collagen type IV (IV-C) in rat serum were measured by radioimmunoassay method. The level of hydroxyproline (Hyp) in liver was detected by chemistry method. The pathological changes of liver tissue were observed by HE and Von-Gieson staining.</p><p><b>RESULT</b>FZJGF could significant decrease the serum activities of ALT and AST, and increase the levels of TP,Alb and ratio A/G. The levels of LN, HA and IV-C were decreased significantly after the treatment using FZJGF. The pathological improvements were observed. FZJGF could markedly alleviate the deposition of collageneous fiber, and reduce the liver pseudoluboli and the fibrosis scores in the liver tissue compared with model group.</p><p><b>CONCLUSION</b>FZJGF can inhibit formation and development of rat hepatic fibrosis induced by the human serum albumin.</p>